Resources>Publications

 

 

Selected recent publications about FAIMS    (2009)

 

 

Rapid Communications in Mass Spectrometry
Volume 23 Issue 13, Pages 1963 - 1969
Published Online: 5 Jun 2009

High-field asymmetric waveform ion mobility spectrometry (FAIMS) coupled with high-resolution electron transfer dissociation mass spectrometry for the analysis of isobaric phosphopeptides

Yue Xuan 1, Andrew J. Creese 2, Julie A. Horner 3, Helen J. Cooper 2

1Thermo Fisher Scientific, Hanna-Kunath-Str. 11, 28199 Bremen, Germany
2School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
3Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA 95134, USA

Abstract
We have applied high-field asymmetric waveform ion mobility spectrometry (FAIMS) to the analysis of the phosphopeptides APLpSFRGSLPKSYVK, APLSFRGpSLPKSYVK, and APLSFRGSLPKpSYVK. The peptides have identical amino acid sequences and differ only in the site of phosphorylation. The results show that FAIMS is capable of at least partially separating these species. Separation was confirmed by coupling FAIMS with high-resolution electron transfer dissociation (ETD) mass spectrometry. Phosphorylation is retained on the ETD peptide fragments thereby allowing assignment of the site of the modification. Co-eluting phosphopeptides which differ only in the site of modification are frequently observed in liquid chromatography/tandem mass spectrometry phosphoproteomics experiments, and therefore these proof-of-principle results have implications for the application of FAIMS in that field. Copyright © 2009 John Wiley & Sons, Ltd.
Accepted for publication: 25 April 2009

 

 

Conference

 

First FAIMS meeting ... Thermo Fisher Scientific Event Showcases New Breakthroughs in FAIMS

News Release from: Thermo Fisher Scientific 07/05/2009
Thermo Fisher Scientific Inc. announced that researchers attending its first ever FAIMS (high-field asymmetric waveform ion mobility spectrometry) meeting in Germany showcased ground-breaking findings. Attendees presented a number of breakthroughs enabled through their use of the FAIMS technology. For example, Michael Blackburn, working with colleagues at sanofi-aventis, described how FAIMS can be used to successfully filter out matrix interferences from mass spectrometry data, leaving a cleaner signal and making it easier to validate bioanalytical methods used in drug development.

Dr. Axel Roemer, an expert in small molecule quantization based at the German contract research organization A&M Labor, presented the keynote lecture. In it he described his company's efforts to improve protein and peptide analysis, and presented the world's first validated method for peptide analysis using FAIMS-based LC-MS (LC-FAIMS-MS). Using peptides in rat serum, Roemer and colleagues have shown that LC-FAIMS-MS chromatograms offer greatly reduced chemical backgrounds compared to those produced without FAIMS. By successfully filtering out complex matrix interferences, which prevent method validation in a traditional LC-MS assay, Roemer reports lower limits of detection as small as 10 ng/mL (100 times smaller than the 1 ug/mL offered by other companies). A&M Labor expects to have even better results at next year's meeting.

FAIMS meeting in Germany

 

 

PHYSICS BOOK

Title: Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS
Author: Alexandre A. Shvartsburg
Publisher: CRC Press, 2008

 

 

Book overview

Over the last decade, scientific and engineering interests have been shifting from conventional ion mobility spectrometry (IMS) to field asymmetric waveform ion mobility spectrometry (FAIMS). Differential Ion Mobility Spectrometry: Nonlinear Ion Transport and Fundamentals of FAIMS explores this new analytical technology that separates and characterizes ions by the difference between their mobility in gases at high and low electric fields. It also covers the novel topics of higher-order differential IMS and IMS with alignment of dipole direction.The book relates the fundamentals of FAIMS and other nonlinear IMS methods to the physics of gas-phase ion transport. It begins with the basics of ion diffusion and mobility in gases, covering the main attributes of conventional IMS that are relevant to all IMS approaches. Building on this foundation, the author reviews diverse high-field transport phenomena that underlie differential IMS. He discusses the conceptual implementation andfirst-principles optimization of FAIMS as a filtering technique, emphasizing the dependence of FAIMS performance metrics on instrumental parameters and properties of ion species. He also explores ion reactions in FAIMS caused by field heating and the effects of inhomogeneous electric field in curved FAIMS gaps. Written by an accomplished scientist in the field, this state-of-the-art book supplies the foundation to understand the new technology of nonlinear IMS methods

Overview
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Earliest publications about FAIMS (prior to 2000)

(1)Title: Separation of ions according to mobility in strong ac fields.
Author: Buryakov, I. A.; Krylov, E. V.; Makas, A. L.; Nazarov, E. G.; Pervukhin, V.V.; Rasulev, U. K.
Publication: Sov. Tech. Phys. Lett., 1991, v17(6), p.446-47.

(2)Title: A new method of separation of multi-atomic ions by mobility at atmospheric pressure using a high-frequency amplitude-asymmetric strong electric field.
Author: Buryakov, I. A.; Krylov, E. V.; Nazarov, E. G.; Rasulev, U. K.
Publication: Int. J. Mass Spectrom. Ion Proc., 1993, v128, p.143-8.

(3)Title: Mass spectrometric characterization of a high-field asymmetric waveform ion mobility spectrometer.
Author: Purves, Randy W; Guevremont, Roger; Day, Stephen; Pipich, Charles W.; Matyjaszczyk, Matthew S.
Publication: Review of Scientific Instruments, 1998, v.69, n.12, p.4094, 12p.

(4)Title: A method of reducing diffusion losses in a drift spectrometer.
Author: Krylov, E. V.
Publication: Tech. Phys., 1999, v44, p.113-6.

(5)Title: Atmospheric pressure ion focusing in a high-field asymmetric waveform ion mobility spectrometer.
Author: Guevremont, Roger; Purves, Randy W.
Publication: Review of Scientific Instruments, 1999, v.70, n.2, p.1370, 14p.

(6)Title: High field asymmetric waveform ion mobility spectrometry-mass spectrometry: an investigation of leucine enkephalin ions produced by electrospray ionization.
Author: Guevremont, Roger; Purves, Randy W.
Publication: Journal of the American Society for Mass Spectrometry, 1999, v.10, p492-501.

(7)Title: Electrospray ionization high-field asymmetric waveform ion mobility spectrometry-mass spectrometry.
Author: Purves, Randy W; Guevremont, Roger
Publication: Analytical Chemistry, 1999, v71, p.2346-2357.

(8)Title: Separation of leucine and isoleucine by electrospray ionization high-field asymmetric waveform ion mobility spectrometry-mass spectrometry.
Author: Barnett, David A.; Ells, Barbara; Guevremont, Roger; Purves, Randy W.
Publication: Journal of the American Society for Mass Spectrometry, 1999, v10, 1279-1284.

(9)Title: Detection of chlorinated and brominated byproducts of drinking water disinfection using electrospray ionization high - field asymmetric waveform ion mobility spectrometry - mass spectrometry.
Author: Ells, Barbara; Barnett, David A.; Froese, Ken; Purves, Randy W.; Hrudey, Steve; Guevremont, Roger
Publication: Analytical Chemistry, 1999, v71, p. 4747-52.

(10)Title: Ion trapping at atmospheric pressure (760 Torr) and room temperature with a high-field asymmetric waveform ion mobility spectrometer
Author: Guevremont, Roger; Purves, Randy W.; Barnett, David A.; Ding, Luyi
Publication: International Journal of Mass Spectrometry, 1999, v193, p. 45-56.